Arabidopsis (Arabidopsis thaliana) AtBIK1 and rice (Oryza sativa) OsRLCK176 are orthologous receptor-like cytoplasmic kinases involved in plant immune signaling. In this Trends in Plant Science Spotlight article, I review several recent studies suggesting that proteasomal turnover of these kinases is regulated by orthologous group IV Ca2+-dependent protein kinases AtCPK28 and OsCPK4, revealing conserved interplay between phosphorylation and ubiquitination in immune homeostasis.
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This month we bid farewell to several labmates. Danalyn joined the lab as a volunteer in early 2016 and continued as a BIOL540 research mentorship student, summer student, and Honours thesis student. She is starting her PhD with Thomas Lahaye at the ZMPB in Tuebingen Germany this summer. Alex began as a volunteer in the fall of 2016 and continued as a summer student and Honours thesis student, and plans to go to law school. Jennifer, who will soon graduate from the Biotechnology Advanced program at St Lawrence College, joined the lab in January of this year as a placement student. For the past months she has kept the lab in tip-top shape, helping everyone with various technical tasks. Cailun joined as a volunteer last summer, mainly working on making in-house DNA ladder using the pPSU vectors. She has recently taken up a position at PnuVax in Montreal. We are all so proud of everything these young scientists have achieved during their time in our group, and we look forward to seeing each of them flourish in their next positions. Farewell and keep in touch!
A capstone experience in the Queen's Biology undergraduate program is the Honours Thesis. This year, I had the pleasure of mentoring two extraordinarily talented young women - 4th-year students Danalyn Holmes and Alexandra Johnson Dingee. Both worked on deciphering the function of the Ca2+-dependent protein kinase CPK28 in Arabidopsis. We and others have shown that this kinase functions in both developmental and immune pathways. Danalyn used epistasis analysis to test if the proteins involved in CPK28-mediated immune signalling are also required for CPK28-mediated developmental signalling. Alex took a trans-complementation approach to test the role of phosphorylation in directing CPK28 function in the two pathways. Together, Danalyn and Alex have made significant contributions to our research program.
Both were recognized with Second Place prizes in the Cell & Molecular Biology section. Very well deserved - congrats on a job well done!
I was honoured to be nominated by the Natural Sciences Research Council of Canada (NSERC) to attend this year's Science and Technology in Society (STS) Forum as part of their Future Leader program. The STS Forum was held at the Kyoto Convention Centre - a unique building surrounded by beautiful gardens. The Forum brings together people from academia, business, and government to discuss the role of science and technology in solving global issues. For 3 days, we heard from panel speakers and participated at round-table discussions on topics such as genome engineering, global health and medicine, artificial intelligence, robotics, disaster prevention, availability of water, and many others. A buffet dinner was held at Kennin-ji Temple, where we were entertained by traditional Japanese performers. This meeting was very different from any other I've attended in the past, and I am thankful to NSERC for sending me.
After the conference, I traveled to Yokohama to visit my friends and colleagues in Ken Shirasu's lab at RIKEN: Yasuhiro Kadota, Shuta Asai, and Thomas Spallek. We all used to work together at the Sainsbury Lab - it was a great reunion and so inspiring to learn what everyone is working on now. The visit ended with a delicious meal of sashimi and shabushabu.
This week the lab took a few days off to retreat in the woods north of Kingston. We stayed overnight at Elbow Lake Environmental Education Centre and enjoyed canoeing, hiking, s'mores, and board games. We also bid farewell to our talented technician Heather, who is off to Ireland for a year to earn her undergraduate degree in Biotechnology.