New publication! "a novel allele of the arabidopsis thaliana macpf protein cad1 results in deregulated immune signaling"
After a long journey, we are excited to share our work on Arabidopsis CAD1 - a protein with some homology to mammalian perforin (!), now published in Genetics. And, we are absolutely thrilled that the journal chose to feature CAD1 on the cover of the April 2021 issue!
This project started while I was a postdoctoral fellow with Cyril Zipfel, and was then championed by Danalyn Holmes, who worked in our lab during her second (volunteer), third (research mentorship and summer student), and fourth year (Hons thesis) at Queen's. Danalyn and I worked closely together on this project and share first-authorship, but this was a true team effort over the years - Sydney Pascetta worked on CAD1 genetics for her Hons thesis, genotyping hundreds of plants to identify cad1 eds1 and cad1 ndr1 double mutants. Melissa Bredow discovered that the mutation we identified in the N-terminus of CAD1 resulted in reduced protein accumulation/stability, and also helped us determine that CAD1 localizes to both the cytosol and the plasma membrane. Irina Sementchokova and Kristen Siegel performed many CAD1-related experiments during their MSc projects, and Kathrin Thor helped with some of the crosses.
Congrats to all authors. This one was in the works for a long, long time and we are so happy that CAD1 has found her home in Genetics.